Formol Titration 2k356r

FORMOL TITRATION NAME: WINDA A ENGKESA NIM: 2015-78-064

Titration of formol is used to show the level of Nitrogen in amino, but it can also be used to measure protein hydrolysis

FORMOL TITRATION OBJECTIVE analysis of nitrogen content in amino by means of formol titration to determine the levels of protein available in the sample. in formol titration, a solution containing a protein made into an acid with an acidic term titrated with a basic effect. The basic solution used is NaOH. because NaOH is included in the baking solution which is an unstable trait in certain conditions it needs to be standardized first by the nature of raw

PRINCIPLE OF FORMOL • the protein solution is neutralized with a base of sodium hydroxide (NaOH), then added formaldehyde to form dimethylol • with the formation of dimethillol the protein amino protein group is bonded and does not affect the reaction between the acid (carboxyl group) with the NaOH base so that the end of the titration can be precisely terminated • indicator used is pp indicator the end of the titration is marked when the pink color changes are lost in 30 seconds

PRINCIPLE OF FORMOL • formol titration is best used for evaluation of the process of protein breakdown (eg in the fermentation of proteins in tempeh, soy sauce, and tauco) • the process of protein hydrolysis is characterized by increased formol titration

ANALSIS PROCEDURE • take 10 ml or 10 g of sample into a 125 ml erlenmeyer and add 20 ml of distilled water and 0.4 ml of saturated potassium oxalate solution (potassium oxalate: water 1: 3) and 1 ml of 1% phenolphthalein. let stand for 2 minutes • sample the sample solution with 0.1 N sodium hydroxide (NaOH) until it reaches a color such as a standard color or pink color • standard color: 10 ml of milk + 10 ml of distilled water + 0.4 ml of saturated potassium oxalate + 1 drops 0.01% indicator rosanilin-chloride

• after the color has been achieved, add 2 ml of 40% formaldehyde solution and re-enrich it with NaOH solution until color as standard color is reached again. record this second titration

• make a blank titration comprising a comparison of 20 ml of distilled water + 0.4 ml of saturated potassium oxalate solution + 1 ml phenolphthalein indicator + 2 ml formaldehyde solution and titration with NaOH

CALCULATION FORMULA % N = (TS-TB) x N NaOH x 14,008 x sample weight (g) x 1000

DF x 100%

TS= titration of the sample TB= titration blank SW= sample weight DF= dilution factor = if according to the feeding procedure DF = 50/10 = 5

REACTION TO FORMOL TITRATION

Advantages Only precisely used to determine a process of solving disadvantages Less precise to determine a particular type of protein

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